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gene chip hybridization oven 640  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gene chip hybridization oven 640
    Gene Chip Hybridization Oven 640, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+chip+hybridization+oven+640/pmc03296652-230-31-30
    Average 86 stars, based on 1 article reviews
    gene chip hybridization oven 640 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Labeling:

    Article Title: Molecular and Cellular Alterations Induced by Sustained Expression of Ciliary Neurotrophic Factor in a Mouse Model of Retinitis Pigmentosa
    Article Snippet: Before hybridization, fractionation of each labeled probe was analyzed (2100 Bioanalyzer; Agilent Technologies, Foster City, CA). .. Each labeled RNA probe (10 μ g) was individually hybridized (Mouse Genome 430–2.0 Arrays in a Gene Chip Hybridization Oven 640) followed by washes in a fluidics station (model 450; all from Affymetrix, Santa Clara, CA). .. Quadruplet arrays were scanned and data were acquired (Scanner 3000 and Gene Chip Operating Software 1.3; Affymetrix).

    Article Title: Actin-binding protein filamin-A drives tau aggregation and contributes to progressive supranuclear palsy pathology
    Article Snippet: .. The labeled DNA was hybridized to CytoScan HD Chip in Gene Chip Hybridization Oven 640 (Affymetrix) with hybridization buffer. .. The chip was washed and scanned with GeneChip Fluidics Station 450 (Affymetrix).

    Hybridization:

    Article Title: Molecular and Cellular Alterations Induced by Sustained Expression of Ciliary Neurotrophic Factor in a Mouse Model of Retinitis Pigmentosa
    Article Snippet: Before hybridization, fractionation of each labeled probe was analyzed (2100 Bioanalyzer; Agilent Technologies, Foster City, CA). .. Each labeled RNA probe (10 μ g) was individually hybridized (Mouse Genome 430–2.0 Arrays in a Gene Chip Hybridization Oven 640) followed by washes in a fluidics station (model 450; all from Affymetrix, Santa Clara, CA). .. Quadruplet arrays were scanned and data were acquired (Scanner 3000 and Gene Chip Operating Software 1.3; Affymetrix).

    Article Title: Regulation of mPGES-1 composition and cell growth via the MAPK signaling pathway in jurkat cells
    Article Snippet: Briefly, 100 ng total RNA was used for cDNA synthesis and biotin-tagged cRNA was produced by the Gene Chip IVT Labeling kit (Affymetrix; Thermo Fisher Scientific, Inc.). .. A total of 15 μg fragmented cRNA, with the controls oligo B2 and eukaryotic hybridization, were hybridized to each GeneChip array at 45°C for 16 h (Affymetrix Gene Chip Hybridization Oven 640) according to the manufacturer protocol. .. Following hybridization, the Gene Chip arrays were washed three times at room temperature and stained with streptavidin phycoerythrin onan (3×; 35°C; 300 sec) with Affymetrix Fluidics Station 450 followed by scanning with the Affymetrix Gene Chip Scanner 30007G.

    Article Title: CLC-3 and SOX2 regulate the cell cycle in DU145 cells
    Article Snippet: The resulting biotin-tagged cRNA was fragmented to 35–200 bases according to the Affymetrix protocols. .. Hybridization was performed at 45°C with rotation for 16 h (Affymetrix Gene Chip Hybridization Oven 640; Thermo Fisher Scientific, Inc.). .. The Gene Chip arrays were washed, stained (streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450 (Thermo Fisher Scientific, Inc.) and scanned on a Gene Chip Scanner 3000 (Thermo Fisher Scientific, Inc.).

    Article Title: Long Non-coding RNAs Expression Profile in HepG2 Cells Reveals the Potential Role of Long Non-coding RNAs in the Cholesterol Metabolism
    Article Snippet: Affymetrix GeneChip ® Human Transcriptome 2.0 ST microarrays were hybridized with 4.7 μg of labeled DNA. .. Arrays were hybridized in the Gene Chip Hybridization Oven 640 (Affymetrix) for 16 hours at 45°C rotating at 60 rpm. .. Washing, staining, and scanning of the arrays were done with the GeneChip Expression Wash, Stain, and Scan Kit (Affymetrix) and the Gene Chip Fluidics Station 450 (Affymetrix).

    Article Title: Actin-binding protein filamin-A drives tau aggregation and contributes to progressive supranuclear palsy pathology
    Article Snippet: .. The labeled DNA was hybridized to CytoScan HD Chip in Gene Chip Hybridization Oven 640 (Affymetrix) with hybridization buffer. .. The chip was washed and scanned with GeneChip Fluidics Station 450 (Affymetrix).

    Article Title: Transcription analysis on response of porcine alveolar macrophages to Haemophilus parasuis
    Article Snippet: Briefly, a total of 1 μg RNA was used for cDNA synthesis and to produce biotin-tagged cRNA with GeneChip IVT Labeling kit (Affymetrix). .. A total of 15 μg fragmented cRNA, with contol oligo B2 and eukaryotic hybridization controls (bioB, bioC, bioD, cre) was hybridized to each GeneChip array at 45°C for 16 hours (Affymetrix Gene Chip Hybridization Oven 640) according to manufacturer's instructions. .. After hybridization, the GeneChip arrays were washed and stained with streptavidin phycoerythrin onan (SAPE) with Affymetrix Fluidics Station 450 followed by scanning with the Affymetrix GeneChip Scanner 3000.

    Article Title: Indoleamine 2,3-dioxygenase 1 activation in mature cDC1 promotes tolerogenic education of inflammatory cDC2 via metabolic communication
    Article Snippet: .. For microarrays, RNA was isolated by NucleoSpin RNA XS (Macherey-Nagel) and amplified with WT Pico System (Affymetrix) and hybridized to Gene Chip Mouse Gene 1.0 ST microarrays (Affymetrix) for 18 hours at 45°C in a Gene Chip Hybridization Oven 640. ..

    other:

    Article Title: Feasibility of the salivary transcriptome as a novel biomarker in determining disease susceptibility.
    Article Snippet: Funding information New Zealand Dental Association Research Foundation Background and Objective: The salivary transcriptome may present as a readily available and noninvasive source of potential biomarkers.. The development of chronic periodontitis is determined by individual patient susceptibility; hence, the aim of this study was to determine the potential of the salivary transcriptome as a biomarker of disease susceptibility using chronic periodontitis as an example.. Material and Methods: Using an Oragene® RNA kit, the total RNA was purified from the saliva of 10 patients with chronic periodontitis and 10 patients without chronic periodontitis.

    Isolation:

    Article Title: Indoleamine 2,3-dioxygenase 1 activation in mature cDC1 promotes tolerogenic education of inflammatory cDC2 via metabolic communication
    Article Snippet: .. For microarrays, RNA was isolated by NucleoSpin RNA XS (Macherey-Nagel) and amplified with WT Pico System (Affymetrix) and hybridized to Gene Chip Mouse Gene 1.0 ST microarrays (Affymetrix) for 18 hours at 45°C in a Gene Chip Hybridization Oven 640. ..

    Amplification:

    Article Title: Indoleamine 2,3-dioxygenase 1 activation in mature cDC1 promotes tolerogenic education of inflammatory cDC2 via metabolic communication
    Article Snippet: .. For microarrays, RNA was isolated by NucleoSpin RNA XS (Macherey-Nagel) and amplified with WT Pico System (Affymetrix) and hybridized to Gene Chip Mouse Gene 1.0 ST microarrays (Affymetrix) for 18 hours at 45°C in a Gene Chip Hybridization Oven 640. ..



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